Pleurothallis is a large genus of myophilous Neotropical orchids with diverse pollination syndromes including both rewarding and deceptive species. SEM was used to analyze the micromorphology of the labellum of several species in Pleurothallis. Physical characteristics such as the size of the labellum, the presence or absence of dried liquid, a sulcus, an apical cavity, or hypochilar projections were used to infer pollination strategies. New ITS sequences were added to existing phylogenetic trees of Pleurothallis subsections to elucidate interspecific phylogenetic relationships. This study specifically documents species with a glenion, and phylogenetically catalogues the glenion in Pleurothallis subsections Ancipitia, Acroniae, and Macrophyllae- Fasciculatae. This study also introduces a previously undescribed tissue type, that we propose calling rugose secretory tissue, that occurs in Pleurothallis subsections Restrepioidia and Elongatia. Preliminary LC-MS data definitively identify large amounts of sugar on the flowers of several Pleurothallis species, strongly suggesting the presence of a nectar reward.